Extraction Kit of Virus DNA / Rna by Membrane Column Method

• Varieties: Test
• Component: Rose-Bengal
• Type: Test
• Pharmacodynamic Influential Factors: Animal Species
• Storage Method: Light Proof
• Transport Package: Box
• Model NO.: 50T
• Specification: 10ml
• Trademark: gention
• Origin: China
• HS Code: 3004209020
• Production Capacity: 100000 Pieces

Product Description

 

Extraction kit of virus DNA / RNA by membrane column method 

PRODUCT NAME:

Extraction kit of virus DNA / RNA by membrane column method.

 

Indications: 

The kit uses optimized extraction buffer system and silica gel adsorption column , which is 

suitable for rapid extraction of virus DNA and RNA from different samples. The extracted 

fragments are relatively complete, with high purity, and can be used in various routine 

experiments of molecular biology. The kit does not use phenol, chloroform and other toxic 

reagents, so it is safe to operate. 

Components:

 

Test method:

1. Sample handling 

1) Tissue treatment: cut small pieces of tissue and add appropriate PBS or 0.9% normal saline, 

grind the tissue thoroughly with electric homogenizer, centrifugate and take the supernatant 

for the second step of lysis. 

2) Cotton swab treatment: transfer the swab wiped in the throat, nose and other parts to a 2ml 

centrifuge tube, add a proper amount of PBS (200μl PBS soaking overnight is better), take the 

supernatant for the second step of lysis. 

3) Cells: direct lysis method: the second step of lysis is carried out immediately. 

2. Take 200μl of the sample after the first step of treatment, add 200μl of buffer GTC, vortex 

shaking for 30s, 56 ºC water bath for 10min. 

3. Add 250μl of national drug grade isopropanol and let stand for 5min at room temperature. 

Transfer all the solutions to the adsorption column, centrifugate at 12000rpm for 1min, 

discard the waste liquid in the collection tube, and put the adsorption column into the 

collection tube again. 

4. Add 600μl wash buffer to the adsorption column, centrifugate at 12000rpm for 1min, 

discard the waste liquid in the collection tube, and then put the adsorption column into the 

collection tube again. 

5. Repeat step 4. 

6.Put the adsorption column in the collection pipe and centrifugate at 12000rpm for 2min. 

Note: the steps should not be omitted to ensure that the residual cleaning solution of the 

adsorption column is removed. 

Put the adsorption column in a clean 1.5ml centrifuge tube, open the cover of the adsorption 

column, leave it at room temperature for 2min, add 30-50μl eluent buffer the adsorption 

column, leave it at room temperature for 2-5min, and centrifugate it at 12000rpm for 2min to 

obtain viral nucleic acid. Short term storage at - 80 ºC
 

Precautions:

All operations should be carried out in strictly with the instructions;Use national drug grade isopropanol, or it will affect the extraction effect; 

When the temperature is low, there may be precipitation in the solution, which must be 

checked before use. If there is precipitation, it should be dissolved by heating in a 56 ºC water 

bath, and it should be used after mixing. After use, tighten the bottle cap ; 

Wear gloves in the experiment and avoid accidental ingestion; 

 

STORAGE:
Store between 2-8ºC. 
Keep out of reach of children.