PCR Test Detection Kits for Big Animals

• Varieties: Test
• Component: Rose-Bengal
• Type: Test
• Pharmacodynamic Influential Factors: Animal Species
• Storage Method: Light Proof
• Package: 50 Kitts/Box
• Model NO.: 50T
• Transport Package: Box
• Specification: 50T
• Trademark: gention
• Origin: China
• HS Code: 3004209020
• Production Capacity: 100000 Pieces

Product Description

 

PCR Test Detection Kits  FOR BIG ANIMALS

PRODUCT NAME:

PCR Test Detection Kits
 

Composition: 

1. Test strip: 1 strip/bag×40 bags (with pipette) 

2. Instruction: 1 sheet 

3. Buffer solution: 1 vial. 

4. Pipette: 40. 

Indications:

Fluorescent quantitative differential diagnosis kit for brucella ruminant (B kit)

Fluorescence quantitative test kit for brucella (wild strain)  (B kit)

Fluorescent quantitative test kit for tuberculosis bacilli  (B kit)

Fluorescent quantitative test kit for tuberous skin disease of cattle  (B kit)

Fluorescent quantitative test kit for small ruminant disease  (B kit)

Fluorescence PCR test kit for African swine fever virus (B kit)

Fluorescence PCR test kit for swine fever virus(generic) (B kit)

Fluorescence PCR test kit for swine fever virus(wild type) (B kit)

Fluorescence PCR test kit for Porcine reproductive and respiratory syndrome virus(generic) (B kit)

Fluorescence PCR test kit for Wild strain of swine pseudorabies virus (B kit)

Fluorescence PCR test kit for Porcine parvovirus (B kit)

Quadruple fluorescence PCR test kit for Swine fever virus/porcine blue ear virus/pseudoravirus/Circovirus type II (B kit)
 

Double fluorescence PCR test kit for Classical American strain of porcine reproductive and respiratory syndrome virus/Highly pathogenic mutant strain of porcine reproductive and respiratory syndrome virus (B kit)

Fluorescent quantitative test kit for Universal foot and mouth disease (B kit)

Triple fluorescent PCR test kit for Swine infectious gastroenteritis virus/Epidemic diarrhea virus/rotavirus (B kit)

Fluorescence PCR test kit for Universal avian influenza virus (B kit)

Triple fluorescent PCR test kit for Avian influenza virus H5/H7/H9 (B kit)

Double fluorescence PCR test kit for Avian influenza virus H7N9 subtype (B kit)

Fluorescence PCR test kit for the H5 subtype of avian influenza virus (B kit)

Fluorescence PCR test kit for the H7 subtype of avian influenza virus (B kit)

Fluorescence PCR test kit for the H9 subtype of avian influenza virus (B kit)

Fluorescence PCR test kit for Newcastle disease virus (B kit)

Fluorescence PCR test kit for avian infectious bronchitis virus (B kit)

Fluorescence PCR test kit for avian infectious laryngotracheitis virus (B kit)

Fluorescence PCR test kit for Avian bursa virus (B kit)

Fluorescence PCR test kit for Chicken marek's disease virus (B kit)

Fluorescence PCR test kit for Avian leukaemia virus (B kit)

Fluorescence PCR test kit for Avian reovirus (B kit)

Fluorescence PCR test kit for Avian encephalomyelitis virus (B kit)

Fluorescence PCR test kit for Duck pestilence virus (B kit)

Fluorescence PCR test kit for Duck hepatitis virus type  (B kit)

Genomic DNA extraction kit for animal tissue(A kit)

Viral DNA/RNA co-extraction kit(A kit)

Membrane column nucleic acid extraction kit(A kit)

Magnetic bead nucleic acid extraction kit(A kit)

Rapid magnetic bead nucleic acid extraction kit(A kit)

 

Method Uses: 

1. Sample handling 

1) Tissue treatment: cut small pieces of tissue and add appropriate PBS or 0.9% normal saline, 

grind the tissue thoroughly with electric homogenizer, centrifugate and take the supernatant 

for the second step of lysis. 

2) Cotton swab treatment: transfer the swab wiped in the throat, nose and other parts to a 2ml 

centrifuge tube, add a proper amount of PBS (200μl PBS soaking overnight is better), take the 

supernatant for the second step of lysis. 

3) Cells: direct lysis method: the second step of lysis is carried out immediately. 

2. Take 200μl of the sample after the first step of treatment, add 200μl of buffer GTC, vortex 

shaking for 30s, 56 ºC water bath for 10min. 

3. Add 250μl of national drug grade isopropanol and let stand for 5min at room temperature. 

Transfer all the solutions to the adsorption column, centrifugate at 12000rpm for 1min, 

discard the waste liquid in the collection tube, and put the adsorption column into the 

collection tube again. 

4. Add 600μl wash buffer to the adsorption column, centrifugate at 12000rpm for 1min, 

discard the waste liquid in the collection tube, and then put the adsorption column into the 

collection tube again. 

5. Repeat step 4. 

6.Put the adsorption column in the collection pipe and centrifugate at 12000rpm for 2min. 

Note: the steps should not be omitted to ensure that the residual cleaning solution of the 

adsorption column is removed. 

Put the adsorption column in a clean 1.5ml centrifuge tube, open the cover of the adsorption 

column, leave it at room temperature for 2min, add 30-50μl eluent buffer the adsorption 

column, leave it at room temperature for 2-5min, and centrifugate it at 12000rpm for 2min to 

obtain viral nucleic acid. Short term storage at - 80 ºC

 

Attention: 

1. Please read the instruction carefully before the test. All the reagents in the product 

only can be use in the test. 

2. The expired products or the aluminium bag broken products can not be used. 

3. Take the detection card out from the refrigerator and stand for to room temperature, 

open the aluminium bag, use as soon as possible, avoid invalid after dampness. 

4. The deionized water, tap water and normal saline can not be use as negative 

control. 

5. The serum sample should be fresh and clear. Contaminate turbid sample, with 

hemolysis sample, with a lot of blood fat sample and very thick blood can not be used.6. The operator can not touch the injection port and chromatography membrane at 

detection port during test procedure. 

7. The waster during the test procedure should be dispose as contamination according 

to related regulations. 

Storage:

1. Stored in a dry place at 2°C~30°C, and should not be frozen. 

2. Avoid direct sunlight. 

3. Shelf life: 24 months from the production date.

 

 
Keep out of reach of children.