Elisa Diagnostic Kit for Antibody to Foot and Mouth Disease Virus O

• Customization: Available
• Type: Diagnosis Product
• Syringe: Diagnosis Product
• Model NO.: 103
• Breathing Pattern: Diagnosis Product
• Animal Anesthesia Machine Control Method: Diagnosis Product
• Transport Package: Carton
• Specification: on your demand
• Trademark: SHENGBO
• Origin: China

Product Description

1.Product name
ELISA Diagnostic Kit for Antibody to Foot and Mouth Disease Virus O                    

1. Principle and Application     

This product consist of FMDV O Ab coated plate,Antibody working solution,HRP Conjugate and other supporting reagent.Using the principle of blocking ELISA to detect of FMDV O-type antibodies in bovine, sheep, porcine serum or plasma.For immune effect evaluation, auxiliary diagnosis, etc.

1. Kit Contents      

FMDV O Ab coated plate	96T×2
HRP Conjugate	11ml
Antibody working solution	11ml
Ag solution	11ml
Positive control	1.5ml
Negative control	1.5ml
Colouration A	11ml
Colouration B	11ml
Stop Solution	11ml
20×Washing Buffer	40ml
Dilution Plate	2 pieces
Specification	1 piece
Sealing plate film	6 pieces

4.  Sample Request              
In this test, fresh bovine, sheep, porcine serum or plasma should be used for detection, and samples with bacterial infection, lipemia, hemolysis and
jaundice should not be used. The samples should
not be stored at room temperature for more than 8 hours; if they are stored for more than 8 hours,
they must be stored at 2 to 8ºC; if they are stored for more than 1 week, they must be stored at -20ºC.



                                                         
5.ELISA protocol

1. Bring the ELISA Kit to room temperature for 30 minutes.The 20X Washing Buffer is diluted 20 times with deionized water or distilled water making it to work lotion(Mix 1 volume of 20×Washing Buffer with 19 volumes of deionized water or distilled water).
2. Sample preparation:

Bovine or sheep serum is diluted 32 times with working lotion for later use. (For example, add 5μl serum to 155μl working lotion, and mix well)
Porcine serum is diluted 16 times with working lotion for later use. (For example, add 5ul serum to 75μl working lotion, mix well)
3)25μl Work lotion and 25μl Diluted sample were added to each Sample well of the plate in turn; Add 50μl of Positive control to two control wells.Add 50μl Negative control to two control wells; Add 50μl of Ag solution to all wells.Mix the solution in the wells by gently rocking the plate.Cover plate with sealing plate film and incubate for 30 minutes at 37ºC.Avoid from light and air vents during incubation.(Note: After adding antigen, the dilution ratio of bovine and sheep serum is 1:128, and the dilution ratio of porcine serum is 1:64.)

1. Discard the solution in the wells.Wash the plate by adding 300μl of work lotion to each well of the plate.Discard the wash solution.Repeat four additional times for a total of five washes. Tap dry on paper towels.

Perform the next step immediately.Do not allow the plate to dry.
5)Add 50μl Antibody working solution to each well of the plate.Cover plate with sealing plate film and incubate for 30 minutes at 37ºC.Avoid from light and air vents during incubation.
6)Repeat step 4).Wash the plate and tap dry on paper towels.
7)Add 50μl of HRP Conjugate to each well of the plate.Cover plate with sealing plate film and incubate for 30 minutes at 37ºC.Avoid from light and air vents during incubation.
8)Repeat step 4).Wash the plate and tap dry on paper towels.
9)Add 50μl Colouration A and 50μl Colouration B to each well of the plate in turn.Mix the solution in the wells by gently rocking the plate.Cover plate with sealing plate film and incubate for 15 minutes at 37ºC.Avoid from light and air vents during incubation.
10)Add 50μl of Stop solution to each well.Gently shake and mix. Measure the Optical Density(OD) value with a microplate reader at a wavelength of 450 nm, and save the results.
6.Result judgment
1) Judgment of the validity of the experiment:
A.The mean OD of P must be≤N×50%
B.The mean OD of N must be≥0.8,
2) Result judgment:
PI(Blocking rate)=(1-S/N)×100%,
PI≥50% : Positive
PI<50% : Negative
*S:Sample OD value
*N:average OD value of Negative control
*P:average OD value of Positive control
7. Storage and expiration date
Store at 2~8ºC, the validity period is 12 months.