Test Kit for Antibodies to Porcine Reproductive and Respiratory Syndrome Virus (Enzyme Linked Immunosorbent Assay- ELISA)

• Varieties: Test
• Component: Rose-Bengal
• Type: Test
• Pharmacodynamic Influential Factors: Animal Species
• Storage Method: Light Proof
• Package: 10bottles/Box
• Model NO.: 96T
• Transport Package: Box
• Specification: 10ml
• Trademark: gention
• Origin: China
• HS Code: 3004209020
• Production Capacity: 100000 Pieces

Product Description

 

Test Kit for Antibodies to Porcine Reproductive and Respiratory Syndrome Virus (Enzyme

Linked Immunosorbent Assay, ELISA)

PRODUCT NAME:

Test Kit for Antibodies to Porcine Reproductive and Respiratory Syndrome Virus (Enzyme

Linked Immunosorbent Assay, ELISA)

 

Indications:

The kit consists of an ELISA plate pre-coated with Porcine Reproductive and Respiratory Syndrome Virus

recombinant protein, enzyme marker and other supporting reagents. Blocking enzyme-linked immunoassay

(ELISA) was used to test Porcine Reproductive and Respiratory Syndrome Virus antibody in pig serum and

plasma samples.

For evaluation of immune effect and auxiliary diagnosis of Porcine Reproductive and Respiratory Syndrome

Virus vaccine.
 

Components:

 

Prepare Sample:

1. Take the wwell animal blood to prepare serum according to the conventional method. The serum is

required to be clear, without hemolysis and pollution. The sample can be stored at 2~8ºC for 1 week, and it

needs to be stored at -20ºC for a long time.

2. Dilute the serum by 40-fold (for example, add 195 μL of sample diluted solution to the dilution plate and

then 5 μL of serum, and mix well). The negative and positive references do not need to be diluted.

3. The concentrated washing solution should be returned to room temperature before use to dissolve the

precipitate. Then dilute with distilled water or deionized water 20 times to make a working washing solution

(for example, 19 volume distilled water or deionized water and 1 volume concentrated washing solution,

mix).
 

Test method:

1. Leave the kit at room temperature for more than 30 minutes before use and return to room temperature

(20-25°C).

2. Take the required amount of ELISA slats, set up 1 well for the blank and 2 wells for the negative/positive

separately, seal the unused slats as soon as possible and store at 2~8ºC.

3. Add 100 μL of the sample diluted solution to the blank wells; add 100 μL of the negative and positive

reference solution to the negative and positive wells respectively; add 100 μL of the diluted sample to each

of the sample well.

4. Mix well, cover the membrane, and place at 37ºC (recommended water bath) in the dark for 30 minutes.

5. Shake off the liquid in the well, add 350μ L of working washing liquid to each well, let it stand for 30

seconds and discard it, repeat washing 5 times, and finally pat dry with clean absorbent paper.

6. Add 100 μL of enzyme marker to each well (except blank wells). Cover the cover film, set at 37°C, and

avoid light for 30 minutes.

7. Wash, same as step 5.

8. Add 50 μL of substrate solution and coloring solution to each well in sequence, mix, cover the cover plate

film, set at 37°C, and avoid light for 10 minutes.

9. Add 50μL of stop solution to each well, mix, and measure the absorbance (OD value) of each well at

450nm (630nm can be used as the reference wavelength). If there is no reference wavelength, use a blank

well to adjusting zero (The absorbance of all wells minus the absorbance of blank).

Results:

1. The sample OD value ≥ 0.38 is positive; the sample OD value between 0.2 and 0.38 is suspicious; the

sample OD value <0.2 is negative.

2. When the result is negative or suspicious, it indicates that the antibody level of the pig is insufficient, and

it is recommended to vaccinate accordingly.

3. Under normal conditions, the negative ≤0.10 and the positive ≥0.6.

 

Caution :

1. Wear gloves and work clothes during the experimental operation, strictly improve and implement the disinfection

and isolation system, and all experimental wastes should be treated as infectious materials.

2. The stop solution is corrosive and should avoid contact with skin and clothing. If it is inadvertently contacted, rinse

immediately with plenty of tap water.

3. When the enzyme plate is taken out from the cold storage, it should be restored to room temperature before opening

the bag. Unused enzyme plates should be stored in a sealed bag with a desiccant.

4. The concentrated washing liquid is easy to crystallize at low temperature, and it needs to be restored to room

temperature to completely dissolve.

5. Each well needs to be filled with liquid during washing to prevent the free enzyme from being washed.

6. The samples used for testing should be kept fresh.

7. The test results must be based on the reading of the microplate reader.

8. Reagent components of different batches should not be mixed.

STORAGE:
Store between 2-8ºC. 
Keep out of reach of children.