Test Kit for Antibodies to Foot and Mouth Disease Virus Asia O

• Varieties: Test
• Component: Rose-Bengal
• Type: Test
• Pharmacodynamic Influential Factors: Animal Species
• Storage Method: Light Proof
• Package: 50kits/Box
• Model NO.: 10ml
• Transport Package: Box
• Specification: 10ml
• Trademark: gention
• Origin: China
• HS Code: 3004209020
• Production Capacity: 100000 Pieces

Product Description

 

Test Kit for Antibodies to Foot and Mouth Disease Virus Asia O(Enzyme Linked Immunosorbent Assay-ELISA)

PRODUCT NAME:

Test Kit for Antibodies to Foot and Mouth Disease Virus Asia O(Enzyme Linked Immunosorbent Assay-ELISA)

 

Indications:

The kit is composed of an ELISA plate pre-coated with Food and Mouth disease O antibody, antibody

working solution, antigen solution enzyme marker and other supporting reagents. Blocking enzyme-linked

immunoassay (ELISA) was used to test foot-and-mouth disease type O antibody in bovine, sheep and pig

serum samples.

For testing foot-and-mouth disease O-type antibodies in the serum of cattle, sheep and pigs and evaluate the

immune effect and assist diagnosis of foot-and-mouth disease O-type vaccine.

Components:

 

Prepare Sample:

In the serum dilution plate, use the sample diluted solution to make a 5-fold dilution of the serum (for

example, add 100 μL of sample diluted solution to 25 μL of serum). The positive reference and negative

reference were also diluted 5 times.

 

Test method:

1. Leave the kit at room temperature for more than 30 minutes before use and return to room temperature

(20-25°C).

2. Take the required amount of enzyme-labeled slats, set up 1 well for the blank and 2 wells for the

negative/positive separately, seal the unused slats as soon as possible and store at 2~8ºC. Wash twice with

diluted washing solution.

3. Add 50 μL of the sample diluted solution to the blank wells; add 50 μL of the negative and positive

reference solution to the negative and positive wells respectively; add 50 μL of the enzyme marker to each

wwell, mix gently by shaking, and incubate at 37°C for 30 minutes.

4. Discard the reaction solution, add 200 μL of washing solution to each well, and do not need to stand.

Shake off the washing solution from the well, repeat washing 5 times, and finally pat dry on clean absorbent

paper.

5. Add 100 μL of substrate solution to each well and coloring for 15 minutes at room temperature in the

dark.

7. Add 50 μL of stop solution to each well and mix well. Within 10 minutes, use 450 nm wavelength (630

nm can be used as reference wavelength) to measure the absorbance (OD value) of each well. If there is no

reference wavelength, use a blank well to adjusting zero (The absorbance of all wells minus the absorbance

of blank)

Results:

The test condition is that the difference between the average OD value of the negative reference well and the

positive reference is ≥0.5.
formula: Y=Sample well OD/ the average OD of negative well

When the result is negative or suspicious, it indicates that the pig antibody level is insufficient, and

recommend to vaccinate accordingly

 

Caution :

1. Wear gloves and work clothes during the experimental operation, strictly improve and implement the disinfection

and isolation system, and all experimental wastes should be treated as infectious materials.

2. The stop solution is corrosive and should avoid contact with skin and clothing. If it is inadvertently contacted, rinse

immediately with plenty of tap water.

3. When the enzyme plate is taken out from the cold storage, it should be restored to room temperature before opening

the bag. Unused enzyme plates should be stored in a sealed bag with a desiccant.

4. The concentrated washing liquid is easy to crystallize at low temperature, and it needs to be restored to room

temperature to completely dissolve.

5. Each well needs to be filled with liquid during washing to prevent the free enzyme from being washed.

6. The samples used for testing should be kept fresh.

7. The test results must be based on the reading of the microplate reader.

8. Reagent components of different batches should not be mixed.

STORAGE:
Store between 2-8ºC. 
Keep out of reach of children.