Brucella Antigen for Rose-Bengal Plate Agglutination Test
Components:
The main component of the product is Brucella S2 strain (CVCC 70502) or S2 and
A99 strains (CVCC 70502 and CVCC 70203).
Assay
Plate agglutination reaction occurs with 1:45 diluted positive serum reference
standard, and does not occur with 1:55 diluted positive serum reference standard.
Appearance
Uniformity suspension. When place for a long time, the upper part is clear, and there
is a little sediment at the bottom.
Indication
Used for Rose-Bengal Plate Agglutination Test to diagnose brucellosis.
Usage:
Take 0.03ml test serum and 0.03ml t antigen, mix, and observe the result within 4
minutes. When appear +,the result is evaluated as positive. For animals with positive
reactions, further complement fixation tests or other diagnostic tests must be
performed.
Criteria for agglutination
++++ Large aggregated areas or particles appear, and the liquid is completely
transparent.
+++ Obvious agglomerated particles, and the liquid is almost completely
transparent.
++ Partly obvious agglomerated particles, and the liquid is slightly transparent.
+ Little agglomeration, and liquid turbidity.
- No coagulation, uniform liquid, turbidity.
Caution :
(1) Shake it well before use. Do not use it when there are contaminated or shaken
clots.
(2) The antigen and serum should be left at room temperature for 30 to 60 minutes
before testing
STORAGE:
Store in a cool place.
Keep out of reach of children.
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